Journal: Journal of Diabetes Research

Article Title: Variations of Lipoprotein(a) Levels in the Metabolic Syndrome: A Report from the Maracaibo City Metabolic Syndrome Prevalence Study

doi: 10.1155/2013/416451

Figure Lengend Snippet: Clinical and biochemical parameters evaluated by gender. The Maracaibo City Metabolic Syndrome Prevalence Study, 2013.

Article Snippet: Likewise, serum hs-CRP levels were quantified employing immunoturbidimetric essays (Human Gesellschaft für Biochemica and Diagnostica MBH), and basal insulin levels were determined after 8 to 12 hours of fasting using DRG International Inc. insulin kits.

Techniques:

Journal: Journal of Diabetes Research

Article Title: Variations of Lipoprotein(a) Levels in the Metabolic Syndrome: A Report from the Maracaibo City Metabolic Syndrome Prevalence Study

doi: 10.1155/2013/416451

Figure Lengend Snippet: Logistic regression models of risk factors for high serum Lp(a) concentration. The Maracaibo City Metabolic Syndrome Prevalence Study, 2013.

Article Snippet: Likewise, serum hs-CRP levels were quantified employing immunoturbidimetric essays (Human Gesellschaft für Biochemica and Diagnostica MBH), and basal insulin levels were determined after 8 to 12 hours of fasting using DRG International Inc. insulin kits.

Techniques: Concentration Assay

Journal: Journal of Diabetes Research

Article Title: Variations of Lipoprotein(a) Levels in the Metabolic Syndrome: A Report from the Maracaibo City Metabolic Syndrome Prevalence Study

doi: 10.1155/2013/416451

Figure Lengend Snippet: Logistic regression model of risk factors for the metabolic syndrome. The Maracaibo City Metabolic Syndrome Prevalence Study, 2013.

Article Snippet: Likewise, serum hs-CRP levels were quantified employing immunoturbidimetric essays (Human Gesellschaft für Biochemica and Diagnostica MBH), and basal insulin levels were determined after 8 to 12 hours of fasting using DRG International Inc. insulin kits.

Techniques:

Journal: Infection and Immunity

Article Title: Complement C3 as a Prompt for Human Macrophage Death during Infection with Francisella tularensis Strain SCHU S4

doi: 10.1128/IAI.00424-17

Figure Lengend Snippet: Complement component C3 mediates enhanced bacterial uptake and macrophage death induction. MDM were infected with SCHU S4 (MOI = 80) in the presence of the indicated sera. *, significant differences in bacterial burden or LDH release when comparing test sera to HS at the indicated times p.i. The results shown are representative of three independent experiments. (B) Deposition of C3 peptides on SCHU S4 (8 × 107 bacteria per lane) was detected via Western blotting. Purified C3 and iC3b proteins served as markers. These results are representative of three independent experiments.

Article Snippet: Commercial human serum (HS) with intact complement activity and C3-depleted HS were purchased from either Quidel (San Diego, CA) or Complement Technology, Inc. (Tyler, TX).

Techniques: Infection, Bacteria, Western Blot, Purification

Journal: Infection and Immunity

Article Title: Complement C3 as a Prompt for Human Macrophage Death during Infection with Francisella tularensis Strain SCHU S4

doi: 10.1128/IAI.00424-17

Figure Lengend Snippet: Engagement of cell surface complement receptors is not sufficient for the induction of macrophage death, despite high cytosolic bacterial load. MDM were first infected at a high MOI with WT SCHU S4 in the presence of C3-depleted HS. This was followed by a second infection with either WT or ΔfevR SCHU S4 in the presence of HS or C3-depleted HS. Significant differences (*) in bacterial burden or LDH release are shown compared to the primary infection of WT in C3-depelted serum alone (no secondary infection). ns, not significant. The results shown are averages from 3 independent experiments in which the average MOI for the primary infection with WT in C3-depleted HS was 1,889. The average MOI in the secondary infection was 189 for WT and 97 for ΔfevR SCHU S4.

Article Snippet: Commercial human serum (HS) with intact complement activity and C3-depleted HS were purchased from either Quidel (San Diego, CA) or Complement Technology, Inc. (Tyler, TX).

Techniques: Infection